By John H. Luft (auth.), James K. Koehler Ph. D. (eds.)
The prior decade has obvious a outstanding elevate within the use of electron microscopy as a researm instrument in biology and medication. therefore, such a lot institu tions of upper studying now boast numerous electron optical laboratories having quite a few degrees of class. education within the regimen use of elec tron optical gear and interpretation of effects is not any longer constrained to some prestigious facilities. nonetheless, temniques used by researm staff within the ultrastructural area became tremendous assorted and intricate. even though a number of rather first-class volumes of electron microscopic temnique at the moment are devoted to the elemental components to be had whim permit the amateur to procure an affordable creation to the sphere, rather few books were dedicated to a dialogue of extra advert vanced temnical features of the artwork. It was once with this view that the current quantity used to be conceived as a convenient reference for staff already having a few heritage within the box, as a knowledge resource for these wishing to shift efforts into extra promising temniques, or to be used as a complicated direction or seminar advisor. subject material has been mosen really at the foundation of pertinence to give researm actions in organic electron microscopy and emphasis has been given these components whim look destined to drastically extend in priceless ness within the close to future.
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Extra info for Advanced Techniques in Biological Electron Microscopy
L) sections, since the lower the specific gravity of the resin, the less interfering electron density there will be between tissue elements in the image. II. Exotic Embedding Materials The literature records a modest amount of effort to examine some new embedding materials, or new variations on older themes. 1. Hydrophilic Gels FERNANDEZ-MoRAN and FINEAN (1957) and GILEV (1958) embedded osmium-fixed tissue in concentrated solutions of gelatin, permitted them to harden by drying and then were able to section the blocks thin enough to examine them in the electron microscope.
When both solutions are combined the actual glycol content of the mixture is 66%. The rest, of course, is by then a much diluted Hanks' solution (experiments have been performed in which the ionic strength was maintained simply by adding an appropriate amount of sodium chloride directly to the otherwise pure glycol. It appears that this has a decidedly deleterious effect). It can be assumed that tissue is well stabilized when it is in 66% glycol, and it is not apt to be damaged by subsequent treatments if these are at all reasonable.
Technical modifications in Maraglas embedding. J. Cell BioI. 17, 203-207 (1963). SPURR, A. : A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31-43 (1969). Sterzing, P. , SCALETTI, J. , NAPOLITANO, L. : Tissue cholesterol preservation: solubility of cholesterol digitonide in ethanol. Anat. Rec. 168, 569-572 (1970). STRANGEWAYS, T. S. , CONTI, R. : The living cell in vitro as shown by dark-ground illumination and the changes induced in such cells by fixing reagents.
Advanced Techniques in Biological Electron Microscopy by John H. Luft (auth.), James K. Koehler Ph. D. (eds.)